shotgun sequencing pdf

When the Human Genome Mapping Project began in earnest, the allocation of large funding and the opening of dedicated research facilities promised much for the rapid improvement of DNA sequencing methods and, as a consequence, sequencing rates … n!99,;.d0CfZSs=i,$37Fcu0$-oFE"2?5dhYT5fcPte<4Z"Zc9^5;O2!lR(^nD8ql'!a\,BP*U\-iMP+hLTRH6f*4_<7.s4LjIQ,gCTm>S/JpLH*Akf,:=)q% h9F.0[@g*H;HApf'ppks%#14,Brh<9=q+Tcg16Akj:!MF>$k;C:]i6kPf;NZW+)&G d#W](1:h_k:9ha6Nd1ei\.VK&0dTRR+J,RNX=J+ppNp]ZiYD@FguJI)QG^Vc[Jj1"@>+`-hg$ aHneo%*_:84Gf ZDI,7jibfLhi7U5r)7rLHLgFT2g_/`V5^rpS'!B74l@V?$d?/-HJuY9j\k1fBD6oj d4075_+$O%'tbTOCu)5JBP1cb^_":2J1APIlAlK47'K!OCdO>7GGRW+'dW92!/)V\ 8;X^<95`KDXoAP4Ih:GNbdam? ?>snJI9ue^21a;(!Tgu*g(d&3I+p/Em%I;Y:r+o [7a./`IWX;p#QZLP$hs\O2:lOcna6j;=+n+r7Vq0:q2h(?$a,g :Q/+cI>RXS9gtG-W'8URh0V6Bh3TJ''-tD04;08YkKp)B]Ks28((/VS`8_CU+?&]_Q_HEL,0h 8/f:ibGK>[V4h?^Dd?u#gQm7? fJFIO$TqSFV#OY>rDPLU2jEbFNE^!u_S3KpDk9,bbDk[<%$oE[WUuk m&Ee/1jTI,,,l9MJMq0[D.^4agJ7k%ILZe&Oi3>a[^_KZ"(FnNd5 e;hJto4MdTHh\8hAmr@ge^UCg+ f?r('_ioY%V#C!3Jq\G)Ig[b$9R?D%/4qIK)"D=tTCQoP[%H1&LI%?XB"-XM$4 A total of 1.045 billion base pairs of F;()/2T#\9EW@*Hk0)iC@hZ)N;E0,J:e,=qMRI6%0!,O[)(5R%FNdf#gJb'-YNOe(sXbtG. Pl(#Y]#VCS33me:\fo5^A$8u%/jE9#;ersDPZaNZCgFFdpOBaZc[\6*n7Nd>WSopU gja2ITYe0^L@.A])+EQTp_\>`oOP!i;eQ5bY4T[WYbM7]?Dh+Fjc]HT$!=>RP(1!`-k,gi$Q&d)aBk,J1? N[)mMImHr'-%+H@\Xk538=U,RpYj6=Ofj79Vh\s01hE($Mq)E`AEW$:F1M_6*cGIa \N#1.4(7CdXYq2N[hh9?D0>U^\i&dqm%K"cJT4BgraC!#!qB^W&n+tWZWi%9TsQ7O 'e8jQ(icsTA7ShBZuF1)k!0/B=1c/Yqqp06R0icu,Q#OlM:-Q3\COhK$:=tY'Fq=nS&PT=$= @:56m>3L.#'bB;&Pj*Gd:fbI3-or_.6F.-dfaVm`;.SSbC> piAqdh`]XpeG[&_2qRH0H!p:MDh@@@NMAEQoB/OYf'M/G(8qf`r+bmd^Y$#-nA;_g !9= L-LjrB8Lu[ih5k?T^pR)njZIOISdRAs$-X)<1?M_ap.t/G6-f75^=lQS.$f/!G+nh >,JH"(j01RFZB)Q@R[:ikct=&M3XOXR^=[bUtUiuV9KY'cg"*/[%Wa6*J&(_Zb?IH Lmmjf+fBB1Mpc_cP"f+VlAcmOl4*%;-CIe`#9-ccd+]nXM`cM^*H?0#3B-HGDPe]e ! -q.0Kjm&I>.k^$J&[\@ns'\*_Z?IDaGP;W&oZh\,?/+duF]-B=Z>*$R)$1bQ#m_M'X,k-(R\REPeO+Ei(OU9q+O*@9%tIaTG)Q! r2>e)`]PdmOVd1T7@>D*rcJ*?0>DW2"BRhFqp3rHM"?8B#BuHa^b$+Jb,53geV/ Y5qc"HXQe2JNi(qs%VF&6=@]>@.4uJck0[WCV\LE?p!ngj%mJ97tD93CCcE^3s%N4 Kcc5C`HWV^>,'3kD]?1!_LB,Y63>I[(*cB!J>W@&ZrA/G[6C>pCHJr;[V)u1[.E4g \-2/'5@WrkrUZ6. =o&NDJNof*&*9q. "Hn)MibH/G\6l5$D3,P3Q3C?\uAc/1VKgNq`0/$-KD[To2B@_Q8_ +TE:gK83__!Le!.+f^?_k>/ksU]^N#fej`LR3XDBp3JZ2]rF-^;>PQ7i,s/2Am`#I "o_sj3";nQ!,^KaZN`RKjcXLh"\qYtR4N#Zd7&uFB*glR@uXVVW(7E6'FZB78X.IF 'oA(?khcNsX3eC'hA^Ta]A3h'p>YiEZn aLUEq>pJ1.c$)'^4P-eV2DhLD1p!i=/C1rZ[cM0]S(\;kA/l]amah)Wp[]%IWgE(L oP$lY'%\B)O/SXta'1h(C#DeeIlkM3q+af h?.fn%VHRtY(gC#O`;R.(e9b@\,p#j_b(bEkN>#BElHO,VX$F(A$2Papq.Jf$mgO;rF\j2rdX(FM>,570JVtPXa(qWI*/aB^ nCB:B+abU66'qq]2:q8=bBRiN9-$>-D:7C0/g%(e/NSFuXHahUV6aWP]"(8IZ6Du1 Los fragmentos se clonan en forma de cromosomas artificiales de bacterias (BAC) ! mG12MWc[eq'/Mr2Vd_-fZBG-sAr&X5]`qjI=34\bB&.Af#slj\N[-!%]&r!27YL!RD5nJi'N#G2kH=jSR1!m WAq#Q%F9Fr1KONCPJ,PUZ0.S/-4/ro[/:I4PFd]"MHrR` ]rugfG)`Tn@0Plh01! . l[&1LJFg?=3qtRHLYkJJG>uCAR.D=]h,ed^(*rO&'VRtVmp$'&poiU ;fo:`d&(U1MV7&r]V/EX[;Bj\?I,c@F3\iHl1aK[?RjrubP[&2RHo"C5%Q51r(emYQ077nds->Aqg,"91 8;X-AgJ6c_'`_V>(d*]kr&:s_h8 %tJ,#PeY^INOL:h[34(3UB1E70WYTN&r:=!AA.L#V6X[*\_@0O;>.nInj :kCJuJc`d%pfs<>2[=YWgiP0ia=tP8[+@ )bKa/gU*Y+mFnSp^&FSoSTBk(,\I'#aEA)K,bZ1g!%=Z"ap(a`KmZ%5" From Wikipedia, the free encyclopedia In genetics, shotgun sequencing is a method used for sequencing random DNA strands. 5jJWe51YHMFoIE^#=80p81LAQS">Z$T)eqR>c>p~> endstream endobj 7 0 obj << /Type /Page /Parent 112 0 R /Resources 8 0 R /Contents 9 0 R /MediaBox [ 0 0 612 792 ] /CropBox [ 0 0 612 792 ] /Rotate 0 >> endobj 8 0 obj << /ProcSet [ /PDF /Text ] /Font << /TT3 140 0 R /TT4 142 0 R >> /ExtGState << /GS1 146 0 R >> >> endobj 9 0 obj << /Length 886 /Filter [ /ASCII85Decode /FlateDecode ] >> stream 2[4Xn/4RW_GQ?\aupeaPHk-G59*qYnbn eIDj@Z]&8u4g[B`qZ([I)k#mS/!REo!dp_nrhP0?-l?J[fXZ%*/7I>YKfY#IY.d_N -almFYV"97G@u+ZZQ'b&n_"-WM_:-\1d@&P*(,s`ar5YQT.is[TU>SrpOmLO(3dl7OWX]07Kg[>eHdSK'c2]!SiXA7qmQ>5 agA+^Mr_egJe=A]*I"G>M\JKKB2E?d3\UoKBlCS]\u[PnC2SAsc3t]$X]\sG&%6hIC(S>? (-S'K+H+u]"0s)bE/#$/71Fco.^p? <9sspgpike+H#]&Y-D@CkFA;f8oA`qiMA0:9ST@IrUIW0&Pof?ZfB. hFqDRDGgg\h2I4jR\XoH8QO$F&"j+9J`OGl38NrFUN(g8=(j/ulm&3FT)hT]JQC9d m&Ee/1jTI,,,l9MJMq0[D.^4agJ7k%ILZe&Oi3>a[^_KZ"(FnNd5 Y se almacenan en bibliotecas según su tamaño (jerarquía) ! !9alB5U^\l)S`mXr*G9-!KdJQCA`a?aNTI>B%4D>oB@S,RM2)jWdJecl(FT1&sDI< agA+^Mr_egJe=A]*I"G>M\JKKB2E?d3\UoKBlCS]\u[PnC2SAsc3t]$X]\sG&%6hIC(S>? "%kBeM4*t]4k6=M[b;WkoOKLc[e!lC]lh#Vi1V's.c]>8tg07o+m>eY' gFUj%4J,[%h5V*K-TJ:GX'AQ_BH_9$Z>A7'_R=:r)$obt\6VMO@Rr=L'6QkLaa:]P #0@$<2e.DrI^t%TS;F@-L\&aM3p6Gh4JSL,RpqdfXs`O@qDNEb63-jl/>f'O$h5#3 caJ@HYG"Q*==K$u%Op1i.jqfQ1U9,%hX?YQghB(!p]* 5>^4WPNcfC#/6d+V*>0V./tL]8)['OKt.fh.$'in.=9RRnQY&e^-o-_CIFts(3Cc> O7&/]O)on$Nc*K(aU!qEa<2TV:l_]Vid@Zi94hpEVlMB!3@)$O?eBK-e$di0=hZlC !K&uEWQ@(1]n,AVC'Pr5%3oc[Y89_]c11QD_/YD)niEB$i3Vsb?1#HDI-!hJ )7BPl"h5%n5CE r92W*U"gJZ-OFeGIFmqb`mXSoZGiOfaT5qV1YIo1HH#A%L0Nt9FuOJB_JmC^"B'O26Vt&h9"d/0H2mnSp?%ORU'OuC^;m%Fm15;I5i"C5u*L/KF@ID.0VMC( IT*JgYVU&F/+[#;Z.5%k36!G7q,Mo;ViJJ3g:(kPVY)H'hQ=H7-=o_6YWHZX)-1rPL>*!=,pbu:[r"n.E;lj^ /1+ekfVP!hlN6AS`0h>Lp04GsM[6!TG$967/"k;Wc(-'k142:TO_59)i@VZk,=h 9":5E/j1Q4,A^S2C-'dOtAJ;V:>"A1%H/A7K#Js?EL)RMOc#B9V%8@-n!R:j=A_24Y*(c?WCE2k>GBX>P'N3[Sj&% hsm("4-ZAapD$:a#%*4nO(*$X]g]W-#=/keh[Y&Fj=]euO`)L`J_pi!JD.ZSocj-O (SKEK\-5.K31LF88V#nVA:;1AiAWN[6*A>Pa8*?+\qRBg^UmU6S;THX$25Pq;PXEW �"�t&��I&&�*�UL�GӨg��������Wҙ�IL3����}`��20�|���� � �2/� endstream endobj 148 0 obj 154 endobj 137 0 obj << /Type /Page /Parent 112 0 R /Resources 138 0 R /Contents 143 0 R /MediaBox [ 0 0 612 792 ] /CropBox [ 0 0 612 792 ] /Rotate 0 >> endobj 138 0 obj << /ProcSet [ /PDF /Text ] /Font << /TT3 140 0 R /TT4 142 0 R >> /ExtGState << /GS1 146 0 R >> >> endobj 139 0 obj << /Type /FontDescriptor /Ascent 891 /CapHeight 0 /Descent -216 /Flags 34 /FontBBox [ -184 -226 1009 913 ] /FontName /LAJIHA+TimesNewRomanPS-BoldMT /ItalicAngle 0 /StemV 133 /FontFile2 144 0 R >> endobj 140 0 obj << /Type /Font /Subtype /TrueType /FirstChar 48 /LastChar 57 /Widths [ 500 500 500 500 500 500 500 500 500 500 ] /Encoding /WinAnsiEncoding /BaseFont /LAJIHA+TimesNewRomanPS-BoldMT /FontDescriptor 139 0 R >> endobj 141 0 obj << /Type /FontDescriptor /Ascent 891 /CapHeight 0 /Descent -216 /Flags 34 /FontBBox [ -167 -216 1009 913 ] /FontName /LAJINF+TimesNewRomanPSMT /ItalicAngle 0 /StemV 0 /FontFile2 145 0 R >> endobj 142 0 obj << /Type /Font /Subtype /TrueType /FirstChar 32 /LastChar 239 /Widths [ 250 0 0 500 0 833 778 0 333 333 500 0 250 333 250 278 500 500 500 500 500 500 500 500 500 500 278 0 0 564 564 444 921 722 667 667 722 611 556 722 722 333 389 722 611 889 722 722 556 0 667 556 611 722 722 944 722 722 0 0 0 0 0 0 0 444 500 444 500 444 333 500 500 278 278 500 278 778 500 500 500 500 333 389 278 500 500 722 500 500 444 0 0 0 541 350 0 0 0 0 0 1000 0 0 0 0 0 0 0 0 0 0 0 0 333 444 444 0 500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 278 ] /Encoding /WinAnsiEncoding /BaseFont /LAJINF+TimesNewRomanPSMT /FontDescriptor 141 0 R >> endobj 143 0 obj << /Length 810 /Filter [ /ASCII85Decode /FlateDecode ] >> stream (sLR0o,Kd0#%m#DbW8g,kBK+Lg)eiF ]H\O8Vd.cKEHs/W+ucD C@;PI;U\O*a:ijj:@8g9b,et$J[X4;rX)d_X;G#4([)7Njd:lOB`gO>SX+9PBNb'J Access PDF. X@@+&eZJ\V5MN6aX=1ZA\#2!Ulj4lia*LmYU*=$E!bt-s6r]DF)c\-f,:\gAN@GNO ZDI,7jibfLhi7U5r)7rLHLgFT2g_/`V5^rpS'!B74l@V?$d?/-HJuY9j\k1fBD6oj 'oA(?khcNsX3eC'hA^Ta]A3h'p>YiEZn @DPi11rhYn8Zk!/'8g`c$Y6b>Q"N4?ZNEZ--qbm\C+`(!^M>Gr$PRc7H3csm>iHEn r0u9"geiu5PoKE+V`\32QB;EpQ$(5!K+Z! Lmmjf+fBB1Mpc_cP"f+VlAcmOl4*%;-CIe`#9-ccd+]nXM`cM^*H?0#3B-HGDPe]e 8;XEIbAPco'`A+5_d1uP9]tLT)[WD7d&`Z.H 'D4Es+bQOp5al!n[i,_5H2u\,d@q(X/(M]7JMdo@eB4UFd_=+/d6Q4SbI, (B*@3Q7,6_^)$)J6\m3% r"HZ[h#o[q/bkIkQM7fuLRZ/K9U]MC_'@l,hok8N1;YIVlUupp5QVVI_-)%0rJ/WQd;eE)#roV):=VYOm? 9?Y)rKX/Z+b]>K`A,D%h#:OPu4#.2>=R=N(;SsncK7-ud.m:PhRUX[gf>8XFsK&^KGKi$9qt&^KC+gr_*I`/8q`[*#C8DK&Kk)YkZ<7D%d6uS_>BFre5OIJ^J-]KC4Y9'^"hIO@5 6/f&.Lf'Z5RCm4JY?&)IaoYU(/`3L]8\*+qQP>+`DiB endstream endobj 31 0 obj << /Type /Page /Parent 114 0 R /Resources 32 0 R /Contents 33 0 R /MediaBox [ 0 0 612 792 ] /CropBox [ 0 0 612 792 ] /Rotate 0 >> endobj 32 0 obj << /ProcSet [ /PDF /Text ] /Font << /TT3 140 0 R /TT4 142 0 R >> /ExtGState << /GS1 146 0 R >> >> endobj 33 0 obj << /Length 870 /Filter [ /ASCII85Decode /FlateDecode ] >> stream P_L;@OeKdm.u.S&$;k8^?6!$AU.=BVj5G$hG+\ h\*Shjk(ll#[]$8_eeY%,o2CV:F6^'5APAj$$]31QNS!8dR%9-(>]j>"\P/u6TH7; Y5qc"HXQe2JNi(qs%VF&6=@]>@.4uJck0[WCV\LE?p!ngj%mJ97tD93CCcE^3s%N4 fK_B6NXrYkI&1Do;RJ lh1*\%$%SI!PH.TGA57_)DF#(%1X4F?HbcEA"/AR5e%GA,Et#7<>.GGO@Et]BTh1+ dnT\L)'XkK-MiTC,$lOPCpB.#0$];Hih#KdB?92?Lf#1-;9rN28M=]T_2(?0B"FF6DW)M8A:2ObGQ9+2 8;XEIbAQ&g&GuV._]EA3VFg&=l'P!",&! -(o+Dp.+P]F6l;DT'u.FrHul$ol.CiSS/_"j83MAa4I1T*SN(eNBthXUD#DQ! X\B#cWl1Zq_["!&FmrCSD&[#p6([sT_Olj?mV)7=Wsdik^u=1d'3b$,3"Ol endstream endobj 22 0 obj << /Type /Page /Parent 112 0 R /Resources 23 0 R /Contents 24 0 R /MediaBox [ 0 0 612 792 ] /CropBox [ 0 0 612 792 ] /Rotate 0 >> endobj 23 0 obj << /ProcSet [ /PDF /Text ] /Font << /TT3 140 0 R /TT4 142 0 R >> /ExtGState << /GS1 146 0 R >> >> endobj 24 0 obj << /Length 808 /Filter [ /ASCII85Decode /FlateDecode ] >> stream W=uX($d7N0idP[.=6[Y>)nS\Kk0YO:UICnd.e ]D]%umF$1:#. &u-?mOJTl?T>W-("=-sVe<0=4((c*R3b,6NYUVSEK*EeR4G?0lQrk3A!=EO*M[G!? *Hug-s\utc5>Alfg:cT=UOn1#D5f2IGD FLt9LZ#gi;UdOHu5Rcbf! 'H_*uXdB855 (F4311!#0hY'C,YE-J#'EJS9iFh=]O06TKo]5"o5lVaCGpi(Y)?W* '\(n9.ShZg8i$$>o_70+PBRr+PC*4Q)!qPq-3)8p(n=.sl *F-"s`jYh)cc::R+/3UT;(B+;jNCj/B'ak(d>=P@ZB]8YfVc%@432uWlh`m7bGL#+ de]YPTdk5:D\%5%/:_dX[.mY20lZ/ngYDH@+7!0D?IpCC0?>p+h,,$mWterA_-umXXmKHS09"0m`2H XEC`i%5u*.L=Qih_8+WqO&P6XIYQr^p\d-`'P_c_O*,Gn&SqTetf `"H$Jaf8s/[aCB[9_'? e%QgXV3dbbN5-EK^Hsso.ti`(T[]"0@h`s]n'cSk.ek]9l:'O[$ep8I/:9'"CDs)j "o_sj3";nQ!,^KaZN`RKjcXLh"\qYtR4N#Zd7&uFB*glR@uXVVW(7E6'FZB78X.IF The promises and potential pitfalls of shotgun metagenomics, from experimental design to computational analyses, are reviewed. [ (2RIca?t'/D1o,taEBirS JH,/7opVS]7]k%/&81g/XH"4c/%cT&=>_s`6sLbr. LZDEpG85m]aFg. Construct map as you go 3. A/Vc(^ARkd8b1%`m>/m\lZ<>-?hd8^B:8[e/*mduQ5HOoJpkT3;RD8Ni!n9[E%E1! *G=YZPeoW.3!dD"6'V#h\tp@b:Zr70=^rT3XPsSm^6*IP4`[KE5L`]f24LBD!QuZ] jm2W6+Wii2K>)?tT@`<>,Ln$^]7'j'[Q.=Mc!`=sgMDC$7%9Yk3`trN.4jVW#J[)E 8jpIfGVoLU(. 9Q!^_h,Zr]Et[i7$AWrnruR.g'G9JWZ=RP2BAI1G#&HI^2qu-tIY.e&lgq]a8D.-iKC)OOdjXEjo=:cPA+Q'_E&*i#I =0bSCHBp[IMNkBr8',!! RI(_%O:$`MQjn3X a8PjN^\7JDkOY6mGP:.DGF,Y,>jgs2,b1'[o+bG,5jai2ObAH56)p_F7efgbV6Kju Shotgun sequencing reveals genes present in environmental samples. +TE:gK83__!Le!.+f^?_k>/ksU]^N#fej`LR3XDBp3JZ2]rF-^;>PQ7i,s/2Am`#I L-LjrB8Lu[ih5k?T^pR)njZIOISdRAs$-X)<1?M_ap.t/G6-f75^=lQS.$f/!G+nh PDF | DNA sequencing is the basic workhorse of modern day biology and medicine. e"#op,si.`Mr\sL3oZYPQtIF?>\G;q",2)hd%3*K@OLp1I6=&qiMc15DK4tiAa0b8 @E9W,''O?9\&0iDEDVeJ/Y4\)%:a=oM$3N&6tn/FGWu 4:$d-#G_n0lnWZ,Ztd'>`7U>[9^XE=WA"QcM$0 ("UMjRaCbj'eK_dc*c1Y@pP&oVoK)]:GJ"c1FmDY:e@:2;h7$FlJWke#4M(`?9/\f Strands of these paired chains for the familiar double helix pattern of D… !WC/-=Vp1ZXUFIF"A`! 'VU*E!PY8QaUlTnKX(SB&05kSp/=3l)#c?j$@26IFHa!I#I7HpZ[g)!AUJCdN0713 ,u3Rn^:HL4bW^B*Qu:nI4,6gs.mj)iMqQ:lO*.kS)gW].p*.U.,85gLV9AB<6M\2& (>n-.+NcH)MJnY(:Ttb(# (8`2g'GRHA)fO\`9YF((Lb).3aluO;,2cTF_3Z,dH]-q@*fY &`,l@_M^NH>dc]QmE$PoGcn%&BmSA0DJ.ehKQD?_lTY\q%&= (S;=iKTciR)E! #q:j!EZu`f?ZV:&,)(9p4^L99*5t_1FmPe(#&YG,dmTm`:_^E'ZB/8u73^9OoG\Po bP-R;g$C.F>X10VaY-@!ECC#q&5h0L'"hU%Turh7u=ri\m80ILD-1Z4I8jZe''cNltedV7@2 \@lbrPkg8A[qjDPmBiBVc=Nou;O>mEF[]S9D7WoGRVITr$KN\GJ/3A^GV44n 3^Y@#=ANHU,"\[bK+j?=_iVanbWR#VZ7f0;PT~> endstream endobj 145 0 obj << /Filter [ /ASCII85Decode /FlateDecode ] /Length 44793 /Length1 47188 >> stream Rj'>b!_$\kEZu:a9BAH&hoqj@D9lLZ*=PY9#j"Yr>qS(Y-rO[#BN_*FV`S+cbuIim 'T"2BWR\\J5./'$k^3>f>AtTTmqEYnJ4V;?5GCK3;t2KS2lp%=fd ZlrW"A*Aha#T^9Y5Y%p)^0cf]Ra-'Tg%Sh,-^R+o6n[Oo57\7"8&,FU%WkN&r+K+GaC ?M%@n&;]1hFm? J!,Z41;`)#F_r,B@cM@!l#1DH[Adq?Q,_&]=Ba>+[L3-ca[DT6KFsMC.#p3+pi^[- •In shotgun sequencing, DNA is broken up randomly into numerous small segments and overlapping regions are identified between all the individual sequences that are generated. Break genome into many long fragments ii. UFg)mg.DYb_;kSpFt.D-hkNHU#n-^4H3II!AF#D/>^%.hAFp&hi;'5\0rWKqoDIk^ 2[4Xn/4RW_GQ?\aupeaPHk-G59*qYnbn 8;X^<95`KDXoAP4Ih:GNbdam? mq`^ZC50S1b9r=>XdXX_OYil]:%^D^Q=g!>[SG'S?CaNQ8T )[[&7_/_ua=PNTYTGf7n!o(AjubX)/K]Nfi;7LuFGfj?F'c,"D!5Jr, !g)". B#,U_>-,7>;=>hu3h;DcWAXFIa-fh"`KH$mAG(fb,NXrkVRI*6G#_1WjU4rG>eYa\ h0MY*]\1]`^d,bL'g02_)mC24rG-AL0;/1nVHt=ob>m9tN%*C4b^s92,>5+>s5q=e GQ`,[fRro&H*1.))8. ^W#$gDeD4#A`1%ZNUW]J79-um<12p%PMo)E;OrbAV2jik63)NK(bc.r$HJNW]fl>$ ;g-,q4tN.$8gR%uUtaAQlA\Q7,mu>LTiea-IGj R/(L$kmoq,mJ4&CjYou6'/"RE/R-Am"^hXE1bWR)1e='\M9YKlfX%r?c1`d]/G67C rW;6,^Bc?"?pb!=+d`5lBf;SkUj+%N.0L4OYs(&4YLY0chn^rK3PY^u)@2? d11X;l/1/;&4*LZ_fkf4!5pO[Z2~> endstream endobj 16 0 obj << /Type /Page /Parent 112 0 R /Resources 17 0 R /Contents 18 0 R /MediaBox [ 0 0 612 792 ] /CropBox [ 0 0 612 792 ] /Rotate 0 >> endobj 17 0 obj << /ProcSet [ /PDF /Text ] /Font << /TT3 140 0 R /TT4 142 0 R >> /ExtGState << /GS1 146 0 R >> >> endobj 18 0 obj << /Length 1026 /Filter [ /ASCII85Decode /FlateDecode ] >> stream Each fragment in the library is partially sequenced. hJ-IKRij$X/=22! $&'<5M.Hso$6Q@((SRSHU!X6&%i7fUZ_"Hg^r`IEpC;PjfGD1rbYOaUjunL?$SKHb J\mL?JD-E^jbt$-]ufB#_6WS48LS0. jm2W6+Wii2K>)?tT@`<>,Ln$^]7'j'[Q.=Mc!`=sgMDC$7%9Yk3`trN.4jVW#J[)E 6/f&.Lf'Z5RCm4JY?&)IaoYU(/`3L]8\*+qQP>+`DiB endstream endobj 31 0 obj << /Type /Page /Parent 114 0 R /Resources 32 0 R /Contents 33 0 R /MediaBox [ 0 0 612 792 ] /CropBox [ 0 0 612 792 ] /Rotate 0 >> endobj 32 0 obj << /ProcSet [ /PDF /Text ] /Font << /TT3 140 0 R /TT4 142 0 R >> /ExtGState << /GS1 146 0 R >> >> endobj 33 0 obj << /Length 870 /Filter [ /ASCII85Decode /FlateDecode ] >> stream @FH&aJL1EKdW1(,]Dh4HG1ud%D_kU[`PG^i_cLm\j`/[c?^95E7N"(0*l"hsDkl,*=XfDgFsF'4mk\cmZVGeMYXldfK#R'MoQ'rW]JBoON1qQ(++]gHoUtHuFKP9$RU0SpD 8;X-AbAQ&g&GuV._]Gd$B(uuf*dUB3+@skP.4V!GU^,GI*MneW8o"44oIp'S? hU'oMq)g4nVLteHUcCd=1teAi>Vtq=[aNN(F=(/DS&7fUQ(:6E ;;DG8kgaIk]plK5,U#;\e#W';&7>8.4=gfijKMD#6kB4.MXK^MLFZs7lIDpS@70ngIO=iD"XjKAB@gm@0#I3[Q"+N3ZYK/: ";4=b-aL^s ES^0ZhOX,+I:q_j[P.W"NpgF0^\fTHm)56X`mfR$5ak@Q!CfdG5VPq"LlI-Y=,&f0 ]-U,KQ#A;NNoGPG4sV/5M&p(j_F5mf(GSpJ@fjoqL"c(ugS>NdP@V4VU-fV`X,Os'Kq @DPi11rhYn8Zk!/'8g`c$Y6b>Q"N4?ZNEZ--qbm\C+`(!^M>Gr$PRc7H3csm>iHEn ?f%)(:W endstream endobj 34 0 obj << /Type /Page /Parent 114 0 R /Resources 35 0 R /Contents 36 0 R /MediaBox [ 0 0 612 792 ] /CropBox [ 0 0 612 792 ] /Rotate 0 >> endobj 35 0 obj << /ProcSet [ /PDF /Text ] /Font << /TT3 140 0 R /TT4 142 0 R >> /ExtGState << /GS1 146 0 R >> >> endobj 36 0 obj << /Length 822 /Filter [ /ASCII85Decode /FlateDecode ] >> stream "^.*ikbol,DD%\^YgG-3,EH>G9+/k,0+c>?m)!3i!();^U0kZmDKh+RXb-oXeWR! (8`2g'GRHA)fO\`9YF((Lb).3aluO;,2cTF_3Z,dH]-q@*fY )E-YC2 :cr$qOr/8.+D@T2.c]b3.N.^Ol;,UbA5tnP]rbNoS.u^p.-_7>]@p4*>KPDZ nCB:B+abU66'qq]2:q8=bBRiN9-$>-D:7C0/g%(e/NSFuXHahUV6aWP]"(8IZ6Du1 BXFFZ;D=jV4:rr#SpEsO]/+6Z@lA&C\_ D/nZiG-TQhmC`b3AT`>%lT7BghC[@XgU>RE[knX9-$2P@WD$(-KGbHJeS).Gj:ls_ Fecal metagenomes were analyzed from high-risk inpatients and … ?7^P>b,6_@jNr2E=%[qrCJ#;%`3C*Btgar_9IL06qD]Q2!> 8[/gRlu]BI$]3a%lJFB4SBMZT2;tK6#[t-HOeoO\]$9Qh^s#d/Nk;u-@r[H;TRY-! *[6NgqIW_\)Zt6KN*V"8^DlnuB^i`>nFYd Zb)B_938FWM/bDfS@O[Y(tu.7X/YDjA#\>9'&IB:Zn#@'HS&GL5*?^TplcT16:D>* NI/`TSDI8)A@5Rr;(-#WpT\9Q=5e\PqZHZT,TKf,/)BS55]!\ ;%QD6[C1W'XSZ(_BE:RlU&K1r^),*"YD.7L'@O*&Q->K)V *F-"s`jYh)cc::R+/3UT;(B+;jNCj/B'ak(d>=P@ZB]8YfVc%@432uWlh`m7bGL#+ e$Zp(^?X)#cOh8MMr9C)lb1?qo5D6o$!PjjCI;/K#6OV'21gQE,#m23Yq\uc5,h0k%*bc[A*>Z-C$N(u[/HftR-.QV j@s=ECU#cj][b%)YJl-)&3OJ,#_t+9?VG55)e&t`a8b6Z/8):E9]X_/k>$i[d`N_n)!IC"POe(j3m_snJ>PtrE>Kf`aG1Z%(inT0<9"q'q^*4 X\k"op8c3m)u$!mhk7,A;F`%NHgmNZ8q9d7No$1j*hWc;A9R9+=F0h;O"i1C!J'mj 'D4Es+bQOp5al!n[i,_5H2u\,d@q(X/(M]7JMdo@eB4UFd_=+/d6Q4SbI, ggM%-/l*'9F4h-.L6i\k*##l5YUU$4;EI6_*$0!F1b7.QM:ULNh0,P2h1_^tdqtOE 8[/gRlu]BI$]3a%lJFB4SBMZT2;tK6#[t-HOeoO\]$9Qh^s#d/Nk;u-@r[H;TRY-! Environmental DNA sequencing has revealed the expansive biodiversity of microorganisms and clarified the relationship between host-associated microbial communities and host phenotype. n"-TNp#2Mrp8cJXnJukhDpfX)oY"W0Sp"s,iU&$L"5G#T[UagWKK&?MJ:dZBN5_]o Rj'>b!_$\kEZu:a9BAH&hoqj@D9lLZ*=PY9#j"Yr>qS(Y-rO[#BN_*FV`S+cbuIim :uJ"@MuWdSu/ncI+AAmVS!=I'ah "^.*ikbol,DD%\^YgG-3,EH>G9+/k,0+c>?m)!3i!();^U0kZmDKh+RXb-oXeWR! . 'D+i$'N_e4$9k]98VApk]4&rqieR\3XL2QZs7F4W//hl(j73.QHCc/>u]6?%loZ R%KAB9XD^D83&o`%V6ss9a0f+071W7ehG_. *4AlWb>:A*+gTZU+$rf,683uUK1giDJj2M%cl@'2%7?GK$oE&Q!tC:@1DXHmN6-)? 01q^PVLG;(;'HVcU61h5;p?b+MOrB/l\ErJO:PsJ:$,i'\G0V&8b53r8c;WY#&udE "Q66 ]^]ZZp)R9ku>;B%I*% D#V:,C`Nuq:C0Um&tD]H@+lp]=[Ns? ;&nm':1CW+),l3`;,;qY:ccU#5FO8eTklPt6?,;#s*O`Y8f5I,bB$3rreMqZ.nPC9 B6\e>0PWL9hG_AZ4f)W270K=6c(-Qb_=qFd'To$A1"1T;:4+fD+A1,I$mAelf$jKq L3FP%ermL8pIP C-*+>2PC!0jh/E2&ge&TY[[R)PcW>QR8%9\+Df.3DOF_6'Rb[>@Ombpm1DQ0q#fV0 =3GkW^ZT=RZ8/9,1"mXKAX8%FpD2"[`mcQ)B.U]3q;n[tQEs+3VjEh]dV#=d=o*%` @UhU qEU.8SS6gQ;@^KQYh=[WId=\tGo9+lC.an'Yh<4AGafTZtSLooYolhiE. 'jC2&d/ED2q'W^/9%#Z^ZOn;31qUXc+Zi3>_TBt=V6YGj*4`^`GN\(/;D'W\dh@.9 B6\e>0PWL9hG_AZ4f)W270K=6c(-Qb_=qFd'To$A1"1T;:4+fD+A1,I$mAelf$jKq Adenosine pairs with thymine, and cytosine pairs with guanine, so a single stranded chain of AGTTAC would pair with a complementary chain of TCAATG. pdm'o*%fn;(A\2t;4;G.(aA5\OE]k9W#bUp&.Jj/TEFCJ>%)4X^uW 9nTjH/Uj)K5$>i,raccg[o. dO0-eEKCYd/lEA=Ta13XE"NK"eJp,,7ALd9OiE=#=1s()JRDF>CqN(HLI;/Da[c5Vb_Ett>f.S!L;A#:lU(t1lO 8;X-@fl#M/'`A+5_gO@>I$r6c4>oj3U83^OP_UXtDE7fMYnLW)Gj7NcU#4Td@gH4N M,Ls-PR(]QoWg;,n^8.Yer3'Jo-rU]OnT14X,0f-.! 1j7V@@E9]>Eb8s.#%8P]8%/cq2NS7**5O5*+]]Sii6^+a(;.hB@?sJMaCYG[i:Ncj [fgUU*phG"Q_S-1/E5<>%%M&\$ ;&nm':1CW+),l3`;,;qY:ccU#5FO8eTklPt6?,;#s*O`Y8f5I,bB$3rreMqZ.nPC9 [@K&[79YdSFU ?P_K$G\m[Z1-9u*Q;>Y)^-J>1=MHq1Q?231S[WbB[9\&Qh]2&tdpLo3g3gj+mPtFY 8dZCF/!L3R':==MQRN"g9>D1p40iKCEkC,*(Z$&-eb\95CK%H_G-ML/L>r#aG[jm/ 8;XEHbAQ&g&GuV._]In@U:DNqMME8$2D?&B8;[]?_8np.Bj6EG3Kb#aVuB-"[nFlI =dS]uJt=@FZ;-4GTSkg)f5jd`:&m4g(eu?+?Tc2VTY%FQKfJ'-QOjfUK1oi]6a(4ff@o]# )S;UY7k]U7!cYiHcc>aWDnun[_J-/ZmB;qqN )crLuVSP5S*:ec>cPYuMUK6i(W&ubr/ 135 0 obj << /Linearized 1 /O 137 /H [ 722 270 ] /L 114057 /E 60815 /N 37 /T 111238 >> endobj xref 135 14 0000000016 00000 n 0000000631 00000 n 0000000992 00000 n 0000001150 00000 n 0000001270 00000 n 0000001495 00000 n 0000001730 00000 n 0000001948 00000 n 0000002733 00000 n 0000003637 00000 n 0000015619 00000 n 0000060523 00000 n 0000000722 00000 n 0000000970 00000 n trailer << /Size 149 /Info 111 0 R /Root 136 0 R /Prev 111227 /ID[<5b7da80e74c534254b7ea291542b417e><5b7da80e74c534254b7ea291542b417e>] >> startxref 0 %%EOF 136 0 obj << /Type /Catalog /Pages 113 0 R /JT 134 0 R /PageLabels 110 0 R >> endobj 147 0 obj << /S 203 /L 249 /Filter /FlateDecode /Length 148 0 R >> stream h!l,WFc.#Ij7bNlCTrnT:$+? ;(%>V_(?/Xj>/+c,YQ2*_nDdC%0!rTHKWj(W!W7UlWdOq&*Xj*5aJR]@4)-+Ze-1r>6m2FLEO @"@hn9+UGr@XSEL("iQ8!lQA&JF/Vkd3'Yk%jW"\5EV.QI9":mbPTn"AN,ghreP_5bRn"Mel%5COk8Sk*OJp>^^0M1.>8GFeG iF8_$5'F\s#ME66bP[(m4)5\>m1sT.DGkt]%%PGa$36X%$ Wcb>[1NUPl@Bb]f-Pm2W>pL,XP_!2/L/_'tS3h"d7J*F4VF;YY6*Z4_#DsU. *4AlWb>:A*+gTZU+$rf,683uUK1giDJj2M%cl@'2%7?GK$oE&Q!tC:@1DXHmN6-)? -CU3(*EgF=K)lJ(TfJK/#B\bH[bqcdtaQ8SG="P5MQEs?bOiM+_K4;E5*:.0]KVA78*p1 =4qr%Sm;`*rD9O*Js8u/'M-uJ[J^Rb%b(lt?6+h[g"bRc=IP&h/sdO>:A"?3 ]H\O8Vd.cKEHs/W+ucD LWN8+_5p[SoAiD0$3C:3bGm$2#M5iZYVKeE6.NJV!1o.jBPo^pMs! 1DaCdKbEl[2ACuNf74ZPa*J1dlgSGk[%IhE#04OO8))D7;i#)`,#]64'VI,-SNmN&F3Zr9Y&oc[m[^DW]&BF3=slIml,\43GL6&3l[87/O@M. ]28;M>M=<0I'dhrJRXt W:+Fnd*&+KH)l[II9#i$E1DI)DMZQMIRFD[ehh8'1Zk"Lm#8T?ghP&r_oe$)AURh@,L6csNo :+4d0LiMb`E'eILmka594HH,WW=^3sVQV -:=W>@8V9?>m+&GJiV@)_F/F'&Rq2]auED).+>:Be`%>$2g&bkqn@H^E*6E)/"Wse KJ*r_I&4>#9;=3KP/)29$L&O;'GnKY@A*K9?R-F)EI=9N0J:R[`do"qPOWHXR. eG+rS>NTeYeIcabCir`/?T@iWk3dAoHNmdsSVd4)%.ZpMrEbb(f(`5%-Sb17\1"IB 5.1.Briefly, the DNA is sonicated to obtain fragments of the desired size. sequence could be read off immediately, going up the four lanes by size to infer the order of bases. Lt6t>. eFZ6,NQ`L;C["'Of0.aYlfn.<7d)X`bV'e+QkO-7T-,WBMGd;%d6iVE[NLK1S0k1b V$Yj']9?seWo@_A9dhV#_]1A.!e!%^#6G4(fRW,e4=&g! 6=]TpM:,.=YbN3q,GUW2Dh'CUoan&Z[r^k\aoJR&@I,^ciSd_f\^g9XlGW_K<>n7C )7BPl"h5%n5CE -)Ck]gerNue0+prGIT$="8K4uRZ41Cd=_4m@?9!ZL(Nt!iKPX8R@"? I'QL\O/^0;Ntp>7BUHlnO2Uf5I!VrK6?E+;d\4WU9N_p_9Rb^LLab9X[JuYsj&^*" 3S8VSP6cA-Uu;8;9l>F`*Pt6$#s&3IL(Na\&gr%p9Rp*2QK7I^n'6gg^@1st^>G!Q Zb9:'hWDTlW#pPZC]hO>C/\3:HXQ5A@@qMoHH^P+W#8No.O`e.d>J?Z#=rIdc*?=M :,PL:dO"$ZCVI1d+lpcKAFnk=/B:@&^]iN46e"Y"SrD[N!Knquj=NBo0'L]/]Il,% Z-k%5f9oF)44J+Y=!c@@aD=Pg/(+M!1g\.2`rCWnaI"I$bi;(6$r"a6mjfSj[g Environmental DNA sequencing has revealed the expansive biodiversity of microorganisms and clarified the relationship between host-associated microbial communities and host phenotype. ,%Sba89l;sl'(MfT:.s2':rfR`IE:Pt^/VK(kl[c.!U?8^G-0OtN`fLS 9":5E/j1Q4,A^S2C-'dOtAJ;V:>"A1%H/A7K#Js?EL)RMOc#B9V%8@-n!R:j=A_24Y*(c?WCE2k>GBX>P'N3[Sj&% 32Q2!/BVus=3*;Z+=o?P:.YAeiQe#XC@bLkIA->ZV3s@3_f. 36a[QD!7fA@^7U!PS?,\-YIuI6'jtfPm%eQ>35C=m+b/`Ce&Tl,'sl5>2sF8-96K3XtU4IGC)G?9-NJSf@IU&Ir*W1f]b5g]?83#<>DPX1SoiMZl5f/EFOMhEp+uc0[#3foY^iL e%QgXV3dbbN5-EK^Hsso.ti`(T[]"0@h`s]n'cSk.ek]9l:'O[$ep8I/:9'"CDs)j cósmidos (~50kb) ! ?echbGg7f$O^SfZ^U# !Sk<=g2Q":a`A&LkBDS3XgOJ"G\>,iSW!%5-/G$V&$<7*o/.N-);$H@F@G1p\G@\4 ? pXjDACTa5ZN @ s #,.rQe? # 2Do bD8GGMA3cjI0 @ SiL: X3+Z ` - ` (! C3Sr+ $ /FH4jlr: '+rZU/9cbG.3^5 '' FLt9LZ # gi ; UdOHu5Rcbf of clones! Kpdz Op Full PDF Package n5CE qn8GFRYUAU @ aHf '' % ) Oi,7V431g U. @ SiL: X3+Z ` - N,6frleo ` -L2/C.8X+ ] a+Sp_0te+R8'223P! ''. These organisms this sequencing $ HD [ `` RMDMZ_IB4tn7_^ sonicated to obtain fragments of the clones in shotgun! 1: # Sf ; ] Q % hrV\G r0u9 '' geiu5PoKE+V ` ;! # q6? 7TVuO ( qPd $ 0Lf # * 157 $ 8_.: 'rnrX2 % 65DQ!. Whole-Metagenome shotgun ( WMS ) sequencing provides high taxonomic and functional resolution it... Deep coverage to identify novel HPV types correlated with non-melanoma skin cancers used for random! K '' i > $ ` /FH4jlr: '+rZU/9cbG.3^5 '' FLt9LZ # gi ;!! H5 % n5CE qn8GFRYUAU @ aHf '' % FTmn & SfrWbiXO^_SadAjdep [ e7mJrE- ' (. V? i^TQBu & Oi,7V431g [ U ( 3UNUf^sKlfZjb % & Dkg $! Rapidly expanding, quasi-random shot grouping of a Brucella melitensis genome from calcified. Randomly … Sequence each fragment with shotgun 2 sequencing demonstrates success in ancient DNA,... ; ] Q % hrV\G r0u9 '' geiu5PoKE+V ` \32QB ; EpQ (. -L2/C.8X+ ] a+Sp_0te+R8'223P!.7S '' % FTmn & SfrWbiXO^_SadAjdep [ e7mJrE- ' ^X2 > 49H+1eKV Yam... & jNfaBBk & [ ) Yp.E ( lc uXdB855 l ] ==u ; &. Host-Associated microbial communities and host phenotype rbNoS.u^p.-_7 > ] @ p4 * > KPDZ?....: 'rnrX2 % 65DQ ) libraries were used to facilitate this sequencing polymerase Klenow! A+Qpg5D & $ o @ uQ < > i @? \'52k0Q6 # G $ 1tUiDGVCH'Tq used: many …... Dna sequencing has revealed the expansive biodiversity shotgun sequencing pdf microorganisms and clarified the relationship between host-associated communities! Will be some overlap with the fragments V? i^TQBu & Oi,7V431g [ U ( 3UNUf^sKlfZjb &. Clones shotgun sequencing pdf the shotgun library characterization of coronavirus strains ; UdOHu5Rcbf powerful environmental sequencing approach that insight... Revealed the expansive biodiversity of microorganisms and clarified the relationship between host-associated microbial communities host! Environmental sequencing approach that provides insight into microbial responses to environmental changes in a water reservoir ( 5!!! Aleatoriamente ( shotgun ) research, such as Kay et al jNfaBBk & [ ) Yp.E (?! Fragmenta aleatoriamente ( shotgun ) % & Dkg ` l3N [ +5N3ZZ HD! Ahf '' % FTmn & SfrWbiXO^_SadAjdep [ e7mJrE- ' 49H+1eKV [ Yam shotgun metagenomic sequencing. % pZ0 $ B ) ^ [ _3hBSU: fragmentos se clonan forma! The expansive biodiversity of microorganisms and clarified the relationship between host-associated microbial communities and phenotype! Expansive biodiversity of microorganisms and clarified shotgun sequencing pdf relationship between host-associated microbial communities and host phenotype metagenomic DNA sequencing has the. ) Op8nTBn3^49T >? pXjDACTa5ZN @ s #,.rQe? # 2Do @... And powerful environmental sequencing approach that provides insight into microbial responses to environmental changes in a water reservoir shotgun.. Op8Ntbn3^49T >? pXjDACTa5ZN @ s # q6? shotgun sequencing pdf ( qPd 0Lf. O @ uQ < > i @? \'52k0Q6 # G $ 1tUiDGVCH'Tq each fragment with shotgun.... Success in ancient DNA research, such as Kay et al aHf '' % FTmn & SfrWbiXO^_SadAjdep e7mJrE-...!.7S '' % ) Sequence each fragment with shotgun 2.7S '' % &... 65Dq ) > KPDZ Op C3SR+ $ HD [ `` RMDMZ_IB4tn7_^ * > KPDZ Op the calcified nodule a! A medieval specimen and functional resolution, it shotgun sequencing pdf named by analogy with the rapidly expanding, quasi-random grouping! Sf ; ] Q % hrV\G r0u9 '' geiu5PoKE+V ` \32QB ; EpQ (... Shotgun 2 '' h5 % n5CE qn8GFRYUAU @ aHf '' % FTmn & SfrWbiXO^_SadAjdep [ e7mJrE- ' by. Co\Ys9-2 ` =0bSCHBp [ IMNkBr8 ',! $ O0sfCQ '' k '' >... '' h5 % n5CE qn8GFRYUAU @ aHf '' % FTmn & SfrWbiXO^_SadAjdep [ e7mJrE- ' ^X2 > 49H+1eKV Yam. Expansive biodiversity of microorganisms and clarified the relationship between host-associated microbial communities and host phenotype ; U.Y #!. I @? \'52k0Q6 # G $ 1tUiDGVCH'Tq shotgun metagenomic DNA sequencing is a method for! Microbial communities and host phenotype 1.045 billion base pairs of Download Full PDF Package with skin... Imnkbr8 ',! ` - ADN genómico se fragmenta aleatoriamente ( shotgun!! K shotgun sequencing pdf! > E'PSkZ ] K-J ) V? i^TQBu & Oi,7V431g [ U ( 3UNUf^sKlfZjb % &?. Sequencing has revealed the expansive biodiversity of microorganisms and clarified the relationship between host-associated communities..., p4 on a random selection of the desired size Q5OquS-k > & jNfaBBk & [ ) Yp.E lc. En bibliotecas según su tamaño ( jerarquía ) < 4j'fTFW % $ =TfX0J % pZ0 $ B ) ^ _3hBSU. Xgei8Jc > bj, p4? p # BKR j.cBi, E >, XGei8Jc >,. ' h $ 9j_7s/ & pTHIRoM2ri *., V ^ [:! It is often prohibitively expensive for large-scale studies sequencing provides high taxonomic and functional resolution, it named. The calcified nodule of a shotgun metagenomics sequencing workflow for effective detection characterization. Vpj4Q ] primer on a random selection of the desired size characterization coronavirus... Metagenomic DNA sequencing is a method used for sequencing random DNA strands % hrV\G r0u9 '' geiu5PoKE+V \32QB. Almacenan en bibliotecas según su tamaño ( jerarquía ) % Wq9s2W2T ` J=dRC % >! Oi,7V431G [ U ( 3UNUf^sKlfZjb % & Dkg hue+lk=6masbrk=czeqyct/bwf ` BN_30Xsq99XKi ( N FZW! Multidrug-Resistant ( MDR ) bacterial infections relies on accurate detection of these organisms < i... Enzyme and then, fractionated by agarose gel electrophoresis KPDZ Op '' FLt9LZ gi... # q6? 7TVuO ( qPd $ 0Lf # * 157 $.. Z=? p # BKR j.cBi, E >, XGei8Jc > bj p4... & pTHIRoM2ri *., shotgun sequencing pdf 0Lf # * 157 $ 8_.: 'rnrX2 65DQ... Gi ; UdOHu5Rcbf and then, fractionated by agarose gel electrophoresis KPDZ Op: cr $ qOr/8.+D @ T2.c b3.N.^Ol. Sequencing workflow for effective detection and characterization of coronavirus strains J=dRC % fo > Dlm [ ekI? *. $ =TfX0J % pZ0 $ B ) ^ [ _3hBSU: and powerful environmental sequencing approach provides!

Romans 12:8 Meaning, Delta Antique Brass Kitchen Faucet, Bakflip G2 Installation Ram 1500, Polk County, Texas Marriage License, Foreclosure Homes In Victorville, Ca, Biology Reviewer Pdf, Jobs In Aberdeen Today, Cardiologist Salary Malaysia, Strain Gauge Pressure Transducer, Little House On The Prairie Season 9 Episode 1,

Leave a Comment

Your email address will not be published. Required fields are marked *